Isolation, Screening and Characterization of Cellolytic Bacteria from Agricultural Fields of Narmada District

The aim of this presented work is to elucidate the screening and characterization of cellulolytic bacteria from soil. Soil samples from different areas of agricultural fields of Narmada district were used for the screening of cellulolytic bacteria by serial dilution and pore plate method. Isolates were additionally characterized by morphological and biochemical tests. Out of forty nine isolates were selected on the basis of clear zone produced 7mm ≥. These five potential isolates were further screened for cellulolytic activity among which three isolates AII3, AI3 and CIII5 exhibited promising activity of cellulase in agar plate assay. Isolation, screening and characterization of isolates for cellulolytic activity provided appreciated and novel enzymes for the alteration of cellulolytic waste into valuable compost.